Shop : Details
Shop
Details
Oktober 2014
Alexander Maaß
Evolutive Ansätze zur Affinitätsmaturierung von Antikörper-Fragmenten durch das immunrelevante Protein Activation-induced Cytidine Deaminase
The scope of this thesis was the in vivo and in vitro mediated maturation of antibodies by the protein AID. By simultaneous maturation and screening in each selection round it was possible to isolate affinity-optimized single clones against specific targets.
His-AID731 was successfully produced recombinantly and the activity was detected. Single- and double strand DNA which coded for thinly affinity shark-antibodies was treated with AID enzyme. The resulting mixture of Uracil-containing DNA was amplified via PCR and the DNA mutant-mixture was integrated in yeast cells. The resulting library was sorted for binding proteins with increased affinity against the target protein by high-throughput screening and affinity-optimized antibodies were isolated against the target protein in selection round two and three by yeast display. This evolutionary affinity maturation of antibodies fragments by the human activation-induced cytidine deaminase (AID) indicates that the antibody optimization from human B cells could be imitated in the S. cerevisiae.
His-AID731 was successfully produced recombinantly and the activity was detected. Single- and double strand DNA which coded for thinly affinity shark-antibodies was treated with AID enzyme. The resulting mixture of Uracil-containing DNA was amplified via PCR and the DNA mutant-mixture was integrated in yeast cells. The resulting library was sorted for binding proteins with increased affinity against the target protein by high-throughput screening and affinity-optimized antibodies were isolated against the target protein in selection round two and three by yeast display. This evolutionary affinity maturation of antibodies fragments by the human activation-induced cytidine deaminase (AID) indicates that the antibody optimization from human B cells could be imitated in the S. cerevisiae.
Schlagwörter: AID; Antikörper; Yeast; Affinitätsmaturierung; Activation-induced Cytidine Deaminase
Verfügbare Online-Dokumente zu diesem Titel
DOI 10.2370/9783844030587
Sie benötigen den Adobe Reader, um diese Dateien ansehen zu können. Hier erhalten Sie eine kleine Hilfe und Informationen, zum Download der PDF-Dateien.
Bitte beachten Sie, dass die Online-Dokumente nicht ausdruckbar und nicht editierbar sind.
Bitte beachten Sie auch weitere Informationen unter: Hilfe und Informationen.
Bitte beachten Sie auch weitere Informationen unter: Hilfe und Informationen.
| Dokument |  | Gesamtdokument | ||
| Dateiart |  | |||
| Kosten |  | 37,35 € | ||
| Aktion |  | Zahlungspflichtig kaufen und anzeigen der Datei - 6,3 MB | ||
| Aktion |  | Zahlungspflichtig kaufen und download der Datei - 6,3 MB | ||
| Dokument |  | Inhaltsverzeichnis | ||
| Dateiart | | |||
| Kosten |  | frei | ||
| Aktion |  | Anzeigen der Datei - 106 kB | ||
| Aktion |  | Download der Datei - 106 kB | ||
Benutzereinstellungen für registrierte Online-Kunden (Online-Dokumente)
Sie können hier Ihre Adressdaten ändern sowie bereits georderte Dokumente erneut aufrufen.
Benutzer
Nicht angemeldet
Export bibliographischer Daten
Shaker Verlag GmbHAm Langen Graben 15a52353 Düren
Mo. - Do. 8:00 Uhr bis 16:00 UhrFr. 8:00 Uhr bis 15:00 Uhr
Kontaktieren Sie uns. Wir helfen Ihnen gerne weiter.
