Header

Shop : Rezensionsexemplar

Shop
Rezensionsexemplar
978-3-8440-3430-1
48,80 €
ISBN 978-3-8440-3430-1
Paperback
186 Seiten
17 Abbildungen
275 g
21 x 14,8 cm
Englisch
Dissertation
März 2015
Frederik Sven Ole Fritzsch
Envirostat technologies for time resolved single cell analysis in contactless controlled microenvironments
The complex biochemical mechanisms in cells have evolved over millions of years of evolution. Most cellular research about intracellular mechanisms of individual cells was analyzed with populations. The averaged data from millions of cells allow no conclusion to e.g. cell-cycle or cell-age dependent mechanisms which makes single cell analysis indispensable. In contrast to a snapshot analysis by flow cytometry, a controlled cultivation of a single bacterium would allow to analyze cellular responses to microenvironmental controlled stimuli. But how to cultivate and analyze a few micrometers large living bacterium in controlled microenvironments? Driven by this pivotal question microfluidic and micro-electronic procedures were developed on glass chips, which enable for the first time contactless isolation and reversible fixation of a single bacterium in stream of medium by negative dielectrophoresis. Especially miniaturized octupole electrodes enabled high resolution and cell type independent manipulations with new analytical possibilities. A critical issue for the fluidic single bacterium manipulations was the connection from macro to microfluidics. Hence, parallel to chip developments a novel "on-tube-seal" was developed. Adhesion analysis allowed the selection of cell repellent coatings and optimized cell isolation. Finally, individual cells of Corynebacterium glutamicum were analyzed in controlled microenvironments. The comparison to population analysis data showed faster growth in a controlled microenvironment and unprecedented bioanalytical resolution when comparing single bacterium cultivations in different microenvironments. In summary, the in this thesis developed technologies enable to study the smallest unit of life, the single cell with novel possibilities.
Schlagwörter: Envirostat; Single Cell Analysis; Microfluidic analysis; Single bacterium analysis; negative dielectrophoresis
Chemical Biotechnology
Herausgegeben von Prof. Dr. Andreas Schmid, Dortmund
Band 20
Bitte senden Sie das Rezensionsexemplar an
Anschrift der Redaktion
Anschrift des Rezensenten
Mitteilung
Sicherheitscode
Datenschutzerklärung
Shaker Verlag GmbH
Am Langen Graben 15a
52353 Düren
  +49 2421 99011 9
Mo. - Do. 8:00 Uhr bis 16:00 Uhr
Fr. 8:00 Uhr bis 15:00 Uhr
Kontaktieren Sie uns. Wir helfen Ihnen gerne weiter.
Social Media